PENGARUH SH-MSCs TERHADAP EKSPRESI GEN INTERLEUKIN 6 (IL-6) DAN CD68 (Studi Eksperimental in vivo Pada Tikus Jantan Galur Wistar Model Sindroma Metabolik )

NURINA, NURINA (2025) PENGARUH SH-MSCs TERHADAP EKSPRESI GEN INTERLEUKIN 6 (IL-6) DAN CD68 (Studi Eksperimental in vivo Pada Tikus Jantan Galur Wistar Model Sindroma Metabolik ). Masters thesis, Universitas Islam Sultan Agung Semarang.

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Abstract

Background: Metabolic Syndrome (MetS) is characterized by obesity, insulin resistance, and chronic inflammation, marked by elevated IL-6 and CD68 levels. Hypoxia-induced Secretome from Mesenchymal Stem Cells (SH-MSCs), which contains growth factors and anti-inflammatory molecules, holds potential as a therapy to reduce inflammation and metabolic dysregulation in MetS. Methods: SH-MSCs secretome was cultured under hypoxic conditions and fractionated using tangential flow filtration (10–100 kDa). Thirty Wistar rats fed a high-fat, high-glucose diet for four weeks were divided into five groups: healthy control (K1), untreated MetS control (K2), positive control receiving Fenofibrate (K3), and treatment groups receiving SH-MSCs secretome intravenously at 150 µL (K4) and 300 µL (K5). IL-6 and CD68 expression in adipose tissue was analyzed using RT-PCR, with statistical analysis performed using ANOVA (p<0.05). Results: K2 exhibited the highest expression of CD68 (2.71 ± 0.07) and IL-6 (1.71 ± 0.11), indicating severe inflammation. K5 showed the lowest expression of CD68 (0.85 ± 0.05) and IL-6 (0.54 ± 0.11), confirming a strong anti-inflammatory effect of high-dose SH-MSCs. Intermediate responses in K3 and K4 suggested a dose-dependent therapeutic effect. SH-MSCs modulated inflammation by inhibiting macrophage activation and pro-inflammatory signaling through IL-10 and TGF-β secretion. Conclusion: SH-MSCs effectively reduce IL-6 and CD68 expression, highlighting their potential as an anti-inflammatory therapy for managing metabolic syndrome. Keywords: SH-MSCs, IL-6, CD68, Anti-inflammatory, Metabolic Syndrome

Item Type:	Thesis (Masters)
Subjects:	R Medicine > R Medicine (General)
Divisions:	Pascasarjana Pascasarjana > Magister Biomedik
Depositing User:	Pustakawan 5 UNISSULA
Date Deposited:	28 Apr 2025 02:36
URI:	http://repository.unissula.ac.id/id/eprint/39328

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